Description

Demultiplex Illumina BCL data using bcl-convert or bcl2fastq

Input

name
description
pattern

ch_flowcell

Channel containing the Illumina SampleSheet CSV describing the samples to be
demultiplexed, together with the directory or tar archive holding the Illumina
BCL data (the sequencer output directory). The meta map carries the flowcell
identifier and lane, e.g. [ id:‘string’, lane: int ].

Structure: [ val(meta), path(samplesheet), path(flowcell) ]

demultiplexer

Which demultiplexer to use, bcl2fastq or bclconvert

Output

name
description
pattern

fastq

Demultiplexed FASTQ files, with read group information added to the meta map.
Samples whose FASTQ files are empty are routed to empty_fastq instead.

Structure: [ val(meta), [ path(fastq) ] ]

*.fastq.gz

empty_fastq

Demultiplexed FASTQ files that contain no reads, separated out so that
downstream processes do not have to handle them.

Structure: [ val(meta), [ path(fastq) ] ]

*.fastq.gz

reports

Demultiplexing reports.

Structure: [ val(meta), path(reports) ]

Reports/*

stats

Demultiplexing statistics (bcl2fastq only).

Structure: [ val(meta), path(stats) ]

Stats/*

interop

InterOp files.

Structure: [ val(meta), path(interop) ]

InterOp/*

logs

Demultiplexing logs (bclconvert only).

Structure: [ val(meta), path(logs) ]

Logs/*

undetermined

FASTQ files holding the reads that could not be assigned to any sample.

Structure: [ val(meta), [ path(fastq) ] ]

Undetermined_S0*_R?_00?.fastq.gz

sav_report

MultiQC report summarising the sequencing analysis viewer (SAV) metrics for
the flowcell.

Structure: [ val(meta), path(html) ]

*.html

sav_data

MultiQC data directory for the SAV report.

Structure: [ val(meta), path(data) ]

*_data

sav_plots

Plots created by MultiQC for the SAV report.

Structure: [ val(meta), path(plots) ]

*_plots